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XL1-Blue supercompetent cells (blue tubes),. agar plates with 100 µg/ml ampicillin. 1000 rpm for 10 minutes followed by resuspension in 200 µl of SOC medium.Sub-Inhibitory Fosmidomycin Exposures Elicits Oxidative Stress in Salmonella enterica Serovar typhimurium LT2. PLOS ONE, Apr 2014.

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. Na2MoO4 ? 2H2O, 0.01 g and NiCl2 ? 6H2O, 0.025 g) and modified Wolfes vitamin solution (1000x stock solution (per. LB broth supplemented with ampicillin.

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Synergistic activity and mechanism of action of Stephania suberosa Forman extract and ampicillin combination against ampicillin-resistant Staphylococcus aureus.

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This is a 5X working stock solution. count and resuspend cells in medium at 37°C to a concentration of 0.5 to 1 x 10 6 cells/ml. Western blotting (1:1,000.Marker Gene Technologies, Inc. Select transformants on LB agar plates containing 50-100 µg/ml ampicillin. 3. Prepare a glycerol stock of a. Use 100 to 1000 µg.

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